|First appearance||Luigi's Mansion|
|Attack method||Spin attack|
|Effective weapon(s)||Poltergust 3000|
Biff can be found lifting weights in the Rec Room, who will (jokingly?) refer to Luigi as a possible punching bag. Deciding that he himself needs a workout, Luigi throws a few punches at a nearby punching bag, which hits Biff, infuriating him. Luigi decides to bludgeon him with the bag 2 more times, knocking him to the ground and exposing his heart. Luigi then vacuumed him up into the Poltergust 3000.